20/12/2018 · They were washed with 1 mL of 0.2 mM CaCl 2 (pH 4.3) in 5 mL glass vials sealed with Parafilm by placing the vials on their sides on a platform shaker (~ 50 rpm) for 30 min. The washing solution was removed, and the root apices were rinsed twice with 1 mL of 0.2 mM CaCl 2 (pH 4.3) (control treatment) or with 0.2 mM CaCl 2 with 60 μM AlCl 3 (pH 4.3) (+Al 3+ treatment).