EPA 524.2 Add 25 mg of ascorbic acid to sample vials before filling. After the vial is filled to overflow, then add one to two drops of 1:1 HCl for each 20 ml of sample volume to a pH of <2. Seal the vial taking care not to trap any air bubbles. Turn vial septa-face down, and shake vigorously for 1 minute. Collect samples in duplicate 40ml VOA