3. Open the vial. 4. Carefully pipet 0.3 mL of sample. 5. Close the vial. 6. Hold the vial by the cap over a sink. Invert gently 2–3 times to mix. The vial will become very hot during mixing. 7. Place the vial into the preheated reactor. Heat in the reactor for 2 hours. 8. Wait about 20 minutes for the vial to cool to 120 °C (248 °F) or