Oxygen Demand, Chemical . Oxygen Demand, Chemical Page3 of 12 5. Prepared Sample: Hold one vial at a 45-degree angle. Use a clean volumetric pipet to add 2.00 mL of sample to the vial. For the 200–15,000 mg/L vials: Use a TenSette ® Pipet to add 0.20mL of sample to the vial. 6. Blank Preparation: Hold a second vial at a 45-degree angle. Use a